Mouse Model – B-cell Lymphoma
Gain access to our engineered mouse models, including CD19-Cre transgenic strain (allowing conditional engineering in B cells), BCL2tracer strain (mimicking sporadic t(14;18) translocation), AID-Cre-ERT2 KI/KO strain (used to monitor memory B cell population), RosaYFP Reporter strain, MLL4 f/F strain, Cas9-GFP Cre strain, and BCL2/KDMT2D knock-out strain (either as a syngenic chimera model or xenograft), and […]
ECCITE-seq
ECCITE-seq combines CRISPR perturbations, RNA sequencing, and protein detection at the single-cell level, enabling integrated analysis of transcriptome, genome, and proteome. This technique provides deep insights into cellular states and regulatory networks, serving as a powerful tool for CRISPR screens, functional genomics, and precision medicine research.
CRISPR Screening Platform
This academic platform proposes to perform CRISPR-based screens, enabling the comprehensive interrogation of the functional consequences of a genomic modification at the whole-genome scale, revealing how genomic alterations in coding or regulatory regions lead to phenotypic changes. The coordinator of the platform is Sandrine Roulland.
Mouse Model
Explore our available mouse models (drug resistant, immuno-competent, or immuno-deficient strains) to investigate lymphoma biology and identify novel drug targets.
3D bioprinting
3D Bioprinting allows the precise creation of complex tissue-like structures using cells and biomaterials. In oncology, it enables modeling of the tumor microenvironment to study cancer mechanisms and test new therapies.
3D bioprinting facility
In vitro modelisation – Memory B cells to plasma cell differentiation
Explore our in vitro model enabling the isolation of memory B cells from blood donors and their differentiation into plasma cells. This system supports the study of protein function through gene expression modulation or drug treatment and is extensively characterized at a molecular and epigenetic levels.
Genome Integrity Analysis: Replication and DNA Damage Detection
Our expertise include DNA combing, detection of cytosolic DNA and detection of DNA damage foci, enabling detailed study of DNA replication and genome stability. Allowing quantification of replication stress, fork resection, and DNA damage at both single-molecule and cellular levels, they help uncover mechanisms of cancer progression, inflammation, and potential therapeutic targets.
Hi-C
By detecting physical interactions between distant DNA segments, Hi-C offers insights into the spatial structure and folding of chromatin. Hi-C serves as a powerful tool for deciphering the principles governing chromatin organization, from the formation of topologically associating domains (TADs) to the spatial compartmentalization of regulatory elements and gene expression domains.
miRNA profiling
miRNA Profiling quantifies and characterizes microRNA expression to study their role in regulating gene expression. It helps identify biomarkers and potential therapeutic targets for cancer treatment.