ECCITE-seq
ECCITE-seq combines CRISPR perturbations, RNA sequencing, and protein detection at the single-cell level, enabling integrated analysis of transcriptome, genome, and proteome. This technique provides deep insights into cellular states and regulatory networks, serving as a powerful tool for CRISPR screens, functional genomics, and precision medicine research.
CRISPR Screening Platform
This academic platform proposes to perform CRISPR-based screens, enabling the comprehensive interrogation of the functional consequences of a genomic modification at the whole-genome scale, revealing how genomic alterations in coding or regulatory regions lead to phenotypic changes. The coordinator of the platform is Sandrine Roulland.
3D bioprinting
3D Bioprinting allows the precise creation of complex tissue-like structures using cells and biomaterials. In oncology, it enables modeling of the tumor microenvironment to study cancer mechanisms and test new therapies.
Reverse Transcriptase Multiplex Ligation-dependant Probe Amplification (RT-MLPA)
RT-MLPA enables simultaneous detection and quantification of multiple RNA targets with high accuracy. In oncology, it is used to analyze gene expression signatures, identify biomarkers, and study disease mechanisms.
Proximity Ligation Assay (PLA)
Proximity Ligation Assay (PLA) enables precise detection and visualization of protein-protein interactions directly within cells. In oncology, it helps uncover signaling pathways, validate therapeutic targets, and reveal mechanisms driving cancer progression.
Immunoglobulin repertoire sequencing (Ig-Rep-Seq)
Immunoglobulin Repertoire Sequencing (Ig-Rep-Seq) deciphers the antibody diversity and immune responseat the single-cell level. This method can be used to study lymphoid malignancies, track immune responses, and guide cancer immunotherapy development.
Genome Integrity Analysis: Replication and DNA Damage Detection
Our expertise include DNA combing, detection of cytosolic DNA and detection of DNA damage foci, enabling detailed study of DNA replication and genome stability. Allowing quantification of replication stress, fork resection, and DNA damage at both single-molecule and cellular levels, they help uncover mechanisms of cancer progression, inflammation, and potential therapeutic targets.
Hi-C
By detecting physical interactions between distant DNA segments, Hi-C offers insights into the spatial structure and folding of chromatin. Hi-C serves as a powerful tool for deciphering the principles governing chromatin organization, from the formation of topologically associating domains (TADs) to the spatial compartmentalization of regulatory elements and gene expression domains.
miRNA profiling
miRNA Profiling quantifies and characterizes microRNA expression to study their role in regulating gene expression. It helps identify biomarkers and potential therapeutic targets for cancer treatment.
ScienceLeadR
ScienceLeadR is an experts’ centric platform dedicated to connect the ecosystem of Life Sciences : Experts, Organizations, Academic Societies, BioTech, MedTech, Pharma Industry, … We provide a unique solution to qualify experts and organizations on all expertise at local or worldwide level. ScienceLeadR qualified more than 3.7 million experts. Based on a proprietary medical ontology, […]