RNA-seq (bulk and single-cell)
RNA sequencing (RNA-seq) is a powerful technique for analyzing gene expression at both bulk and single-cell levels. It reveals RNA abundance, splicing, and modifications, providing insights into cellular function, tissue organization, and disease mechanisms. Bulk RNA-seq profiles large cell populations, while single-cell RNA-seq uncovers cellular heterogeneity and rare cell types, aiding biomarker discovery, understanding drug […]
Single-Cell Analysis Technologies
Our cutting-edge platforms include scRNAseq, scATAseq, spatial transcriptomics, scBRC-seq, scTCRseq, and ECCITE-seq. By leveraging these advanced technologies, we investigate the heterogeneity within cell populations, uncover rare cell types, and decipher the molecular mechanisms underlying development, disease, and immune responses. Our integrated approach enables comprehensive profiling of individual cells, driving transformative discoveries in fields ranging from […]
Spatial Transcriptomics and Proteomics
By capturing the spatial distribution of RNA and proteins in situ, our transcriptomic and proteomic platforms provides a comprehensive view of gene and protein expression respectively, within their native tissue context. Based on multiple approaches (from single-cell spatial-Omics to imaging mass spectrometry) and our lymphoid neoplasm expertise, we explore spatially defined expression patterns, cell-cell interactions, […]
Transgenesis platform
Our transgenesis platform enables the generation of customized mouse models through precise genetic modifications, including gene overexpression, knock-in mutations, and conditional gene targeting. These models provide powerful tools to study gene function, disease mechanisms, and therapeutic strategies.
CRISPR Screening Platform
This academic platform proposes to perform CRISPR-based screens, enabling the comprehensive interrogation of the functional consequences of a genomic modification at the whole-genome scale, revealing how genomic alterations in coding or regulatory regions lead to phenotypic changes. The coordinator of the platform is Sandrine Roulland.
DNA methylation – ERRBS
Enhanced Reduced Representation Bisulfite Sequencing (ERRBS) maps DNA methylation at single-nucleotide resolution to study epigenetic regulation. It helps reveal cancer-associated epigenetic changes and identify potential biomarkers and therapeutic targets.
Epitranscriptomics
Our epitranscriptomics pipeline uses LC-MS/MS to detect and quantify RNA modifications, providing insights into RNA regulation. We use this innovative platform to unravel the intricacies of epitranscriptomic modifications during critical cellular processes such as B to plasma cell differentiation, lymphomagenesis, and myelomagenesis. It helps to shed light on the underlying molecular pathways driving disease progression […]
BH3 profiling toolkit
Our BH3 mimetic toolkit measures early drug-induced changes in proapoptotic signaling, revealing shifts in antiapoptotic dependencies. It helps to identify novel drug targets and predictive biomarkers, and offers to assess cellular fate across diverse experimental settings.
DNA-RNA immunoprecipitation sequencing (DRIP-seq)
DRIP-seq maps DNA-RNA hybrids (R-loops) across the genome to study their roles in gene regulation and genome stability. It helps reveal mechanisms of transcription, DNA repair, and chromatin organization, providing insights relevant to disease and potential therapeutic strategies.
Okazaki fragments sequencing (OK-seq)
OK-seq enables researchers to map the genome-wide locations of proteins involved in DNA replication and replication fork directionality. By unraveling the spatial organization of replication machinery and Okazaki fragments, OK-seq offers insights into the coordination and regulation of DNA synthesis during genome replication. This approach provides information for understanding genome stability and cellular proliferation.