CRISPR Screening Platform
This academic platform proposes to perform CRISPR-based screens, enabling the comprehensive interrogation of the functional consequences of a genomic modification at the whole-genome scale, revealing how genomic alterations in coding or regulatory regions lead to phenotypic changes. The coordinator of the platform is Sandrine Roulland.
Mouse Model – Natural killer/T-Cell lymphoma
Gain access to this mouse model (p53-KO x JARID-KO-flox x CD4-cre) for investigating NK/T-cell lymphoma biology, identifying novel drug targets and testing for drug efficacy and toxicity
Mouse Model – Peripheral T-cell lymphoma (PTCL)
Gain access to this mouse model to investigate PTCL biology, identifying novel drug targets and testing for drug efficacy and toxicity
CAR-NK cells generation and characterization
Our expert teams specialize in the precise engineering of CAR-NK cells, tailoring them to recognize specific tumor antigens with high precision. Through functional characterization, we validate the potency and specificity of these engineered cells, ensuring their efficacy in targeting cancer cells while sparing healthy tissues.
T-cell lymphoma cell lines
Explore our available T-cell lymphoma cell lines (>21). Conduct in-depth experiments to unravel the biology of T-cell lymphoma and identify novel drug targets.
DNA methylation – ERRBS
Enhanced Reduced Representation Bisulfite Sequencing (ERRBS) maps DNA methylation at single-nucleotide resolution to study epigenetic regulation. It helps reveal cancer-associated epigenetic changes and identify potential biomarkers and therapeutic targets.
Epitranscriptomics
Our epitranscriptomics pipeline uses LC-MS/MS to detect and quantify RNA modifications, providing insights into RNA regulation. We use this innovative platform to unravel the intricacies of epitranscriptomic modifications during critical cellular processes such as B to plasma cell differentiation, lymphomagenesis, and myelomagenesis. It helps to shed light on the underlying molecular pathways driving disease progression […]
DNA-RNA immunoprecipitation sequencing (DRIP-seq)
DRIP-seq maps DNA-RNA hybrids (R-loops) across the genome to study their roles in gene regulation and genome stability. It helps reveal mechanisms of transcription, DNA repair, and chromatin organization, providing insights relevant to disease and potential therapeutic strategies.
Okazaki fragments sequencing (OK-seq)
OK-seq enables researchers to map the genome-wide locations of proteins involved in DNA replication and replication fork directionality. By unraveling the spatial organization of replication machinery and Okazaki fragments, OK-seq offers insights into the coordination and regulation of DNA synthesis during genome replication. This approach provides information for understanding genome stability and cellular proliferation.
3D bioprinting
3D Bioprinting allows the precise creation of complex tissue-like structures using cells and biomaterials. In oncology, it enables modeling of the tumor microenvironment to study cancer mechanisms and test new therapies.